QIAGEN

Tetra·His Antibody, BSA-free

For sensitive detection of His-tagged proteins with HHHH epitopes

· Detection of C-terminal, N-terminal, and internal His tags

· Highly specific and sensitive detection

The Tetra·His Antibody is supplied as 100 µg of mouse monoclonal antibody (IgG1), as BSA-free, lyophilized powder. It can detect C-terminal, N-termal, and internal His tags.

The Tetra·His Antibody has negligible cross-reactivity with crude E. coli, yeast, insect, or mammalian cell lysates.

Feature	Specifications
Detection	Secondary antibody required
Epitope detected	HHHH
Sensitivity in dot blots -chemiluminescent substrate-	10 pg
Sensitivity in dot blots -chromogenic substrate-	0.5 ng
Sensitivity in western blots - Chemiluminescent substrate	50 pg
Sensitivity in western blots -chromogenic substrate-	2 ng
Substrate for blot detection	Dependent on secondary antibody
Substrates for assay procedure	Dependent on secondary antibody
Tag	His tag

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