QIAGEN

RNeasy Protect Bacteria Mini Kit

For stabilization and purification of up to 100 µg total RNA from bacteria

·         Immediate in vivo RNA stabilization and protection

·         Reliable gene expression and gene-profiling data

·         No need for liquid nitrogen, dry ice, or phenol

·         Ready-to-use, high-quality total RNA in minutes

·         No CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Protect Bacteria Mini Kit includes RNAprotect Bacteria Reagent for stabilizing RNA in bacterial samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology.

Efficient disruption of bacterial samples can be achieved using the TissueLyser II. The kit can be automated on the QIAcube.

Feature	Specifications
Applications	PCR, qPCR, real-time RT-PCR, microarray
Elution volume	30-100 µl
Format	Spin column
Main sample type	Bacteria
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Sample amount	15 -100 µg
Stabilization	Yes
Technology	Silica technology
Time per run or per prep	20 minutes
Yield	8-70 µg

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