QIAGEN

RNeasy Protect Animal Blood System

For collecting and stabilizing animal blood and purifying RNA and miRNA

·         Reliable stabilization of transcript levels at blood collection

·         Convenient collection and storage of small blood volumes

·         Rapid purification of high-quality total RNA

·         Effective recovery of miRNA

The RNeasy Protect Animal Blood System provides a complete solution for preparing high-quality RNA from animal blood. Blood from mouse, rat, and other small animals is conveniently collected in RNAprotect Animal Blood Tubes, which contain a reagent that immediately stabilizes cellular RNA.

Total RNA is then purified using the RNeasy Protect Animal Blood Kit. Please note that the RNeasy Protect Animal Blood Kit and the RNAprotect Animal Blood Tubes are ordered separately.

Alternatively, the RNeasy Protect Animal Blood Kit can be used to obtain microRNA (miRNA), either in a total RNA fraction or in a separate small RNA fraction.

For purification of total RNA containing miRNA, Buffer RWT (cat. no. 1067933) is also required. For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit (cat. no. 74204) is also required.

Feature	Specifications
Applications	Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume	14 µl
Format	Spin column
Main sample type	Tissue, cells
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Total RNA
Sample amount	100 µl or 500 µl animal blood
Technology	Silica technology
Yield	Rat blood (500 ?l): 10-30 µg; Mouse blood (100 ?l): 4-8 µg

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QIAGEN RNeasy Protect Animal Blood System

For collecting and stabilizing animal blood and purifying RNA and miRNA

QIAGEN

RNeasy Protect Animal Blood System