QIAGEN

RNeasy Plus Mini Kit

For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns

·         Unique gDNA Eliminator columns avoid the need for DNase

·         Efficient removal of genomic DNA

·         Highly reproducible yields of RNA in minutes

·         High-performance RNA for sensitive applications

The RNeasy Plus Mini Kit integrates fast, convenient purification of up to 100 µg RNA with effective elimination of genomic DNA.

Cell or tissue lysates are spun through gDNA Eliminator spin columns to remove genomic DNA. Total RNA is then purified using RNeasy Mini spin columns.

The kit can be automated on the QIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

For smaller samples, the RNeasy Plus Micro Kit (spin-column binding capacity of 45 µg RNA) is also available

Feature	Specifications
Applications	Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing
Elution volume	30-50 µl
Format	Spin column
Integrated removal of genomic DNA	Yes
Main sample type	Tissue, cells
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Total RNA
Sample amount	30 mg (<700 µl)
Technology	Silica technology
Time per run or per prep	25 minutes
Yield	Varies

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