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RNeasy Mini Kit
QIAGEN
For purification of up to 100 µg total RNA from cells, tissues, and yeast
- Fast procedure delivering high-quality total RNA in minutes
- Ready-to-use RNA for high performance in any downstream application
- Consistent RNA yields from small amounts of starting material
- No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 g RNA.
Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube.
For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 µg RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.
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Feature |
Specifications |
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Applications |
Northern, dot, and slot blotting, end-point RT-PCR,
quantitative, real-time RT-PCR, array analysis, next-generation
sequencing |
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Elution volume |
30-100 µl |
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Format |
Spin column |
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Main sample type |
Tissue, cells |
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Processing |
Manual
(centrifugation) |
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or
protein |
Total RNA |
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Sample amount |
0.5-30 mg |
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Technology |
Silica technology |
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Time per run or per
prep |
20 minutes |
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Yield |
Varies |
ventas@labequim.com.mxTelefonos: 01 (222) 219 88 15, 274 50 72, 274 50 73
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