QIAGEN

RNeasy Fibrous Tissue Mini Kit

For purification of up to 100 µg total RNA from fiber-rich tissues

·         Optimized protocols for fibrous tissues

·         High yields of total RNA

·         High-quality RNA for all downstream applications

·         Removal of genomic DNA using DNase I

The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA. The kit can be automated on the QIAcube.

Fibrous tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

For larger samples, the RNeasy Fibrous Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.

Feature	Specifications
Applications	PCR, real-time PCR, microarray
Elution volume	30-100 µl
Format	Spin column
Main sample type	Fibre-rich tissue samples
Processing	Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Sample amount	0.5-30 mg
Technology	Silica technology
Time per run or per prep	55 minutes
Yield	5-12 µg

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