QIAGEN

RNeasy FFPE Kit

For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections

·         Novel method to overcome formalin crosslinking

·         Efficient release of RNA without compromising integrity

·         Streamlined protocol providing RNA in just 85 minutes

The RNeasy FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions reverse formaldehye modification of RNA.

In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase and DNase Booster Buffer for optimized removal of genomic DNA contamination.

RNeasy MinElute spin columns enable purification of total RNA with elution volumes of as low as 10 ?l. Purification can be fully automated on the QIAcube.

Feature	Specifications
Applications	PCR, qPCR, real-time RT-PCR, microarray
Elution volume	14-30 µl
Format	Spin column
Main sample type	FFPE tissue samples
Processing	Manual (centrifugation), automated (QIAcube)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Sample amount	15 µm to 410µm sections
Technology	Silica technology
Time per run or per prep	70 minutes
Yield	Varies

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