QIAGEN

REPLI-g UltraFast Mini Kit

For ultrafast highly uniform whole genome amplification from small or precious samples

·         Fast 1-1.5 hour whole genome amplification

·         Obtain unlimited DNA for downstream applications

·         Reproducible amplification from a variety of starting materials

·         Highly stable DNA suitable for long-term storage

A lack of sufficient quantities of genomic DNA for genomic analysis can be overcome by global amplification of all DNA within a sample (whole genome amplification).

The REPLI-g UltraFast Kit includes REPLI-g UltraFast DNA Polymerase, which enables highly uniform and accurate whole genome amplification from small samples in just 60-90 minutes, resulting in typical DNA yields of 7 ?g per 20 ?l reaction.

A variety of starting materials can be used, including genomic DNA, fresh or dried blood, buccal swabs, fresh or frozen tissue, and cells. The DNA generated is suitable for direct use in a range of genetic analyses.

Feature	Specifications
Amplification	Whole genomic DNA
Applications	Genotyping, microarray, PCR, real-time PCR
Denaturation step	Alkaline
Maximum input volume	10 ng DNA, 0.5 µl whole blood, ~300 cells/µl
Minimal pipetting volume needed	1 µl
Quality assessment	No
Reaction time	60-90 minutes
Reaction volume	20 µl
Samples per run; throughput	Mid
Starting amount of DNA	>10 ng purified genomic DNA
Starting material	Genomic DNA, blood, cells
Technology	Multiple Displacement Amplification (MDA)
Yield	~10 µg

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