QIAGEN

QIAGEN Plasmid Plus Giga Kit

For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA

· Rapid purification of up to 12 samples in 40-50 minutes

· Easy vacuum-based parallel processing

· Low elution volumes for highly concentrated DNA

· Very low endotoxin levels due to special wash buffer

· Suitable for all applications

The QIAGEN Plasmid Plus Giga Kit enables ultrafast, large-scale purification of up to 10 mg of highly pure plasmid DNA.

The use of a vacuum manifold allows purification of up to 12 samples in parallel, reducing the need for hands-on time.

Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation.

The QIAGEN Plasmid Plus Giga Kit also features a novel wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.

Feature	Specifications
Applications	Transfection of most cell lines, cloning, enzymatic restriction, in-vitro translation/transcription, sequencing
Culture volume/starting material	2500 ml
Final DNA concentration	? 1 µg/µl
Plasmid type	Plasmids, cosmids
Processing	manual (centrifugation and vacuum), parallel processing
Samples per run; throughput	1-12 samples per run
Technology	Advanced Silica technology (non-chaotrophic), Endotoxin reduction
Time per run or prep per run	50 min
Yield	up to 10 mg

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