QIAGEN

QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits

For purification of up to 50 µg transfection-grade plasmid DNA in 96-well format

·         Transfection-grade plasmid DNA with endotoxin levels <1 EU/µg DNA

·         DNA purity comparable to anion-exchange technology

·         High yields (up to 50 ?g)

·         Convenient handling and simple procedure

·         Vacuum, centrifugation, and automated processing options

QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits provide a novel method for plasmid preparation in 96-well format.

Samples can be conveniently processed using the QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System.

Due to the proprietary binding chemistry, up to 50 ?g of transfection-grade plasmid DNA per well can be obtained from up to 5 ml of an E. coli culture.

Endotoxin levels are reduced to levels below 1 EU/µg DNA due to a special endotoxin reduction buffer.

Feature

Specifications

Applications

Transfection into sensitive cell lines, preparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing etc

Culture volume/starting material

1.25-5 ml culture volume

Plasmid type

High-copy, low-copy, cosmid DNA

Processing

QIAvac 96, centrifuge, automated

Samples per run; throughput

48-96 samples per run

Technology

QIAGEN Plasmid Plus technology

Time per run or prep per run

50 minutes

Yield

15-50 µg

Servicios

Equipo laboratorio

Material laboratorio

Audio y Video

Material de curación

Servicio Técnico

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