QIAGEN

Ni-NTA Spin Kit

For fast, small-scale purification of His-tagged proteins, includes spin columns, reagents, buffers, and control plasmid

·         Up to 300 ?g His-tagged protein per column in as few as 15 minutes

·         Purification under native and denaturing conditions

·         Up to 95% homogeneity in one step

·         Ready-to-use spin columns for rapid automated or manual processing

Ni-NTA silica combines Ni-NTA with a macroporous silica support material optimized to suppress nonspecific hydrophobic interactions.

The Ni-NTA Columns in the Spin Kit provide Ni-NTA silica in a convenient microspin format for easy preparation of multiple samples in parallel.

They provide a simple method for functional screening of engineered proteins, selection of clones expressing full-length translation products, and comparison of expression levels. Each spin column can purify up to 300 µg of His-tagged protein.

Like all Ni-NTA matrices, Ni-NTA spin columns can be used for one-step protein purification under native or denaturing conditions.

The Ni-NTA Spin Kit is a complete kit for spin purification of His-tagged proteins.

Feature	Specifications
Applications	Proteomics
Bead size	16-24 µm
Binding capacity	Up to 300 µg per spin column
Gravity flow or spin column	Spin column
Processing	Automated
Scale	Small scale
Special feature	Up to 95% homogeneity in one step
Start material	Cell lysate
Support/matrix	Macroporous silica
Tag	6xHis tag

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QIAGEN Ni-NTA Spin Kit

QIAGEN

Ni-NTA Spin Kit