For purification of His-tagged proteins by gravity-flow chromatography
- One-step purification from crude lysate to >95% pure protein
- High binding affinity and high capacity
- Choice of purification under native or denaturing conditions
- Precharged, ready-to-use matrices for any scale of purification
- Automated purification and assay protocols
Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag.
Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity.
Cleared cell lysates are loaded onto the matrices. His-tagged proteins are bound, and other proteins pass through the matrix.
After washing, His-tagged proteins are eluted in buffer under native or denaturing conditions.
|
Feature |
Specifications |
|
Applications |
Proteomics |
|
Bead size |
45-165 µm |
|
Binding capacity |
Up to 50 mg/ml |
|
FPLC |
Yes |
|
Gravity flow or spin
column |
Gravity flow |
|
Processing |
Manual/Automated |
|
Scale |
Large scale |
|
Special feature |
Batch and column
purification |
|
Start material |
Cell lysate |
|
Support/matrix |
Sepharose CL-6B |
|
Tag |
6xHis tag |
|
Yield |
100 µg - 100
mg |
