QIAGEN

HiSpeed Plasmid Maxi Kit

For ultrafast purification of up to 750 µg transfection-grade plasmid or cosmid DNA

·         Less than 60 minutes prep time

·         Lysate clearing and isopropanol precipitation without centrifugation

·         No risk of DNA pellet loss during precipitation

·         Up to 750 µg yield of high-copy plasmid DNA

·         LyseBlue for optimum lysis and maximum DNA yield

HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation.

The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Feature	Specifications
Applications	Transfection, cloning, sequencing, gene silencing
Culture volume/starting material	150 µl - 250 ml culture volume
Plasmid type	High-copy, cosmid DNA
Processing	Manual (filtration)
Samples per run; throughput	1 sample per run
Technology	Anion-exchange technology
Time per run or prep per run	<60 min
Yield	<750 µg

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