QIAGEN

EndoFree Plasmid Giga Kit

For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA

·         Less than 0.1 EU/µg DNA achieved

·         Fast procedure, with integrated endotoxin removal

·         High yields of high-copy plasmid DNA

·         LyseBlue for optimum lysis and maximum DNA yield

EndoFree Plasmid Kits provide rapid, anion-exchange-based endotoxin-free plasmid DNA purification. QIAfilter Cartridges enable fast lysate clearing by filtration.

DNA yields up to 500 ?g (Maxi), 2.5 mg (Mega), or 10 mg (Giga) are achieved.

The purified DNA exceeds the purity obtained by 2 x CsCl gradient centrifugation and is suitable for advanced transfection-grade applications.

Feature	Specifications
Applications	Transfection, gene therapy
Culture volume/starting material	2.5 liters culture volume
Plasmid type	High-copy, low-copy, cosmid DNA
Processing	Manual (vacuum)
Samples per run; throughput	1 sample per run
Technology	Anion-exchange technology
Time per run or prep per run	310 min
Yield	<10 mg

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