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QIAGEN

AllPrep DNA/RNA/Protein Mini Kit

 

 

For simultaneous purification of DNA, RNA, and protein from cells and tissues

·         Maximal recovery of each analyte from precious samples

·         Time savings through short, streamlined workflow

·         Comparable data as analytes come from same sample

·         No need for organic solvents such as phenol and acetone

 

The AllPrep DNA/RNA/Protein Mini Kit allows simultaneous purification of DNA, RNA, and protein from the same cell or tissue sample.

The kit can be used in combination with Allprotect Tissue Reagent, which delivers immediate stabilization of DNA, RNA, and protein in tissue samples for long-term storage without freezing.

Tissues can be efficiently disrupted using a TissueRuptor or TissueLyser system .

Feature

Specifications

Applications

PCR, RT-PCR,real-time PCR, real-time RT-PCR, Southern-,Northern-,Western Blotting, Microarrays, Genotyping,1D and 2D gel electrophoresis

Elution volume

RNA: 30 - 50µl, DNA: 100 - 200µl

Format

Spin column

Main sample type

Easy-to-lyse tissue or cells

Processing

Manual (centrifugation)

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

Total RNA, genomic DNA and western-grade proteins

Sample amount

up to 30 mg tissue or 1 x 10e7 cells

Technology

Silica technology, Protein precipitation

Time per run or per prep

60 min

Yield

varies, depends on sample type

 

 

 

 

 

 

 

 

 

 

 

 

 

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Copyright © 2004 - www.labequim.com.mx ventas@labequim.com.mx

25 ote. #217 San Pedro Cholula, Puebla, Pue; Col. San Pedro Mexicaltzingo;

TEL/FAX: 01 (222) 2198815, 274 5072, 274 5073

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