QIAGEN

pQE-TriSystem Vector

For parallel expression of His-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a single construct

No need for time-consuming subcloning procedures

Obtain post-translational modifications in insect or mammalian cells

One construct provides efficient expression in three expression systems

The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems.

There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells.

Feature

Specifications

All three reading frames provided No

Expression In vivo

Expression species E.coli, mammalian & insect cells

In-frame cloning necessary Yes

N- or C-terminal tag C-terminal tag

Tag 6xHis tag

Tag removal sequence No

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