QIAGEN

pQE-30 Xa Vector

For expression of His-tagged proteins containing a Factor Xa Protease recognition site

· Easy cloning of a construct with a Factor Xa Protease recognition site

The pQE-30 Xa vector is similar to pQE-30, but also encodes a Factor Xa Protease recognition site, which is bracketed by the His tag coding region on the 5' side and the multiple cloning site on the 3' side.

5'-end cloning using the blunt-end StuI restriction site allows insertion of the gene of interest directly behind the Factor Xa Protease recognition site, without any intervening amino acid codons.

Factor Xa Protease cleaves off the His tag peptide behind the arginine residue of the protease recognition site (IEGR) and results in a recombinant protein free of any vector-derived amino acids at the N-terminus.

After digestion, Factor Xa Protease can be removed using Xa Removal Resin.

Feature	Specifications
All three reading frames provided	No
Expression	In vivo
Expression species	E. coli
In-frame cloning necessary	Yes
N- or C-terminal tag	N-terminal tag
Tag	6xHis tag
Tag removal sequence	Factor Xa recognition site

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