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QIAGEN

 

miRNeasy Mini Kit

 

For purification of microRNA and total RNA from tissues and cells

 

·         Effective purification of miRNA and total RNA

·         Efficient enrichment of miRNA and RNAs <200 nucleotides

·         High-purity RNA suitable for all downstream applications

·         Protocols for copurification or isolation of separate fractions

The miRNeasy Mini Kit enables purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards from all types of animal tissues and cells, including difficult-to-lyse tissues.

Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required).

The miRNeasy Mini Kit is used for low-throughput RNA purification using spin columns. Copurification of miRNA and total RNA using the miRNeasy Mini Kit can be automated on the QIAcube.

Feature

Specifications

Applications

PCR, qPCR, real-time RT-PCR, microarray

Elution volume

30 µl

Format

Spin column

Main sample type

Tissue, cells

Processing

Manual (centrifugation or vacuum)

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

miRNA, total RNA

Sample amount

50-100 mg

Technology

Silica technology

Yield

Varies

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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