For purification of microRNA and total RNA from formalin-fixed, paraffin-embedded tissue sections

·         Effective purification of miRNA and total RNA

·         Novel method to overcome formalin crosslinking

·         Efficient release of RNA without compromising integrity

·         Streamlined protocol providing RNA in just 85 minutes

The miRNeasy FFPE Kit enables purification of total RNA that includes RNA from approximately 18 nucleotides upwards from formalin-fixed, paraffin-embedded (FFPE) tissue sections. The kit provides recovery of usable RNA fragments, including miRNA and other small RNAs, for applications such as quantitative, real-time RT-PCR.

Feature	Specifications
Applications	PCR, qPRC, real-time RT-PCR, microArray
Elution volume	14-30 µl
Format	Spin column
Main sample type	FFPE tissue samples
Processing	Manual (protocol for automated processing on QIAcube available)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	miRNA, total RNA
Sample amount	up to 4 sections, each with a thickness up to 10 µm and a surface area up to 250mm^2 (automated protocol on QIAcube: up to 2 sections)
Technology	Silica technology
Yield	Varies

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