QIAGEN

mericon Sample Preparation Kits

For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods

·         Modified CTAB procedure ensures faster sample processing

·         Efficient isolation of even fragmented DNA from processed foods

·         Fast and efficient lysis of Gram-negative and Gram-positive bacteria

·         No carryover of PCR inhibitors from complex food matrices

mericon Sample Preparation Kits are designed to enable the extraction of high-quality nucleic acids from a wide range of starting materials, such as pathogen enrichment cultures (mericon DNA Bacteria and Bacteria Plus Kits) and a variety of raw and processed foods (DNeasy mericon Food Kit).

The carryover of PCR inhibitors from these complex food matrices is minimized.

The isolated, pure DNA is suitable for downstream applications, such as real-time PCR ingredient authentication, pathogen detection, or GMO DNA detection and quantification using mericon PCR Assay Kits.

Feature	Specifications
Applications	PCR, real-time, genotyping
Elution volume	100 µl (mericon DNA Bacteria and DNA Bacteria Plus Kit), 150 µl (DNeasy mericon Food Kit)
Format	spin columns (DNeasy mericon food Kit), microcentrifuge tube (DNA Bacteria Kit), icrocentrifuge tube with glass beads (DNA Bacteria Plus Kit)
Main sample type	fod, e.g. chocolate, ketchup, cereals (DNeasy mericon Food Kit), bacterial culture from food (DNA Bacteria Kit and DNA Bacteria Plus Kt)
Processing	manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	DNA
Sample amount	1 ml culture (DNA Bacteria and Bacteria Plus Kit), 2 g (DNeasy mericon Food Kit)
Technology	silica technology (DNeasy mericon food Kit), boiling lysis (DNA Bacteria Kit), disruption with glass beads (DNA Bacteria Plus Kit)
Time per run or per prep	20 minutes - 1 hour (DNeasy mericon Food Kit)
Yield	depends on DNA contents of the sample

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