QIAGEN

Xa Removal Resin

For removal of Factor Xa Protease after protease digestion

· Specific and efficient removal of Xa protease

· Results in high purity of the target proteins

After purification of a 6xHis-tagged protein expressed using pQE-30 Xa vector and cleavage of the 6xHis tag using Factor Xa Protease, the protein of interest can be repurified in two steps.

Xa Removal Resin binds Factor Xa Protease in a batch procedure, and is removed by centrifugation.

Cleaved 6xHis-tag peptides and undigested 6xHis-tagged protein are captured by Ni-NTA affinity chromatography, the purified protein of interest being in the flow-through.

Feature	Specifications
Applications	Proteomics
Binding capacity	50 µl bed volume/4U enzyme
Gravity flow or spin column	Spin column
Processing	Manual
Start material	Protein
Support/matrix	Xa removal matrix

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