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QIAGEN

TurboCapture 96 mRNA Kit

 

 

For rapid, easy, high-throughput mRNA purification from cells

 

·         mRNA purification in 96-well format

·         Few protocol steps and minimal hands-on time

·         mRNA purification and cDNA synthesis in the same well

·         Standard plates compatible with robotic workstations

The TurboCapture 96 mRNA Kit uses unique oligonucleotide immobilization technology to provide a fast and simple procedure for purifying mRNA.

 

 Cell lysates are added to the wells of a TurboCapture plate (96 wells), and mRNA is allowed to hybridize to the immobilized oligo-dT in each well.

 

 Contaminants are washed away, and the isolated mRNA is then either used directly in cDNA synthesis or eluted for use in downstream applications. For higher throughput purification in 384-well format, the TurboCapture 384 mRNA Kit is available.

Feature

Specifications

Applications

PCR, cDNA synthesis

Elution volume

80 µl

Format

96-well plate

Main sample type

Cultured cells

Processing

Manual (centrifugation)

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

mRNA

Sample amount

80 µl

Stabilization

Yes

Technology

Oligonucleotide immobilization technology

Time per run or per prep

30 minutes - 1.5 hours

Yield

Varies

 

 

 

 

 

 

 

 

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