QIAGEN

TAGZyme pQE Vector Set

For expression of proteins optimized for His-tag removal using TAGZyme enzymes

·         Expressed His-tag optimized for removal by TAGZyme enzymes

·         High-level expression of N-terminally His-tagged proteins

·         Expression levels up to 50% of total cellular protein

Both TAGZyme pQE vectors encode an N-terminal 6xHis tag that possesses a sequence optimized for DAPase digestion, and give excellent protein expression rates.

For proteins that do not contain an intrinsic DAPase stop point, TAGZyme pQE-1 encodes for a glutamine residue between the His-tag sequence and the protein sequence, which serves as a DAPase stop point when converted to pyroglutamate by the action of excess Qcyclase Enzyme.

For proteins that contain an intrinsic DAPase stop point, TAGZyme pQE-2 allows the removal of the complete N-terminal tag irrespective of the cloning site of the DNA insert. Both vectors contain an ampicillin resistance gene, a Col E1 origin of replication, a T5 promoter, lac operators and a ribosome-binding site. The pQE-2 vector carries alacl^q repressor gene.

Feature	Specifications
All three reading frames provided	No
Expression	In vivo
Expression species	E. coli
In-frame cloning necessary	Yes
N- or C-terminal tag	No tag
Tag	6xHis tag
Tag removal sequence	TAGZyme System necessary

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