QIAGEN

TAGZyme Qcyclase/pGAPase Enzymes

For removal of N-terminal His tags from TAGZyme pQE vector-expressed proteins with non-native stop point

·         Highly specific exoproteolytic activity prevents internal cleavage

·         Efficient tag removal: >95% in just 30 minutes at 37°C

·         High-purity end products

·         Complete removal of contaminates by Ni-NTA method

The recombinant TAGZyme Qcyclase and pGAPase Enzymes are used in conjunction with TAGZyme DAPase Enzyme for the removal of His tags from proteins with an inserted glutamine residue expressed using the TAGZyme pQE-1 vector.

The glutamine residue is converted in the presence of excess Qcyclase enzyme to pyroglutamate, which serves as a DAPase stop point.

The pyroglutamate residue at the N-terminus of the target protein is removed by the action of pGAPase enzyme.

TAGZyme Qcyclase and pGAPase Enzymes both carry an uncleavable C-terminal His tag for their removal from reaction mixtures by subtractive IMAC, enabling recovery of pure, detagged target protein.

Feature	Specifications
Applications	Production of therapeutic proteins, protein structure determination using NMR or X-ray crystallography
Efficiency of Tag removal	>95%
Removal of fragments and uncleaved proteins	Yes
Special feature	Complete removal of contaminants
Tag removal	Exoproteolytic
Time	30 minutes

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