·
Highly specific exoproteolytic activity prevents internal
cleavage
·
Complete solution for His-tag
removal
·
Efficient tag removal: >95% in just 30 minutes at
37°C
·
High-purity end products
·
Subtractive Ni-NTA chromatography to remove all
contaminants
The TAGZyme Kit contains TAGZyme
Enzymes, reagents, and Ni-NTA Agarose sufficient for highly
specific and efficient His-tag removal from up to 10 mg His-tagged
protein.
The kit can be used for His-tag removal from proteins
containing an intrinsic DAPase stop point (expressed using the TAGZyme
pQE-2 vector, and processed using TAGZyme DAPase Enzyme), or from
proteins that contain an engineered glutamine stop point (expressed
using the TAGZyme pQE-1 vector, and processed using TAGZyme DAPase,
Qcyclase, and pGAPase Enzymes).
To choose
the right vector for your application, contact QIAGEN Technical
Services or your local
distributor.
