QIAGEN

TAGZyme DAPase Enzyme

For the removal of N-terminal His tags from proteins expressed using TAGZyme pQE vectors

·         Highly specific exoproteolytic activity prevents internal cleavage

·         Efficient tag removal: >95% in just 30 minutes at 37°C

·         High-purity end products

·         Completely removal of contaminants by Ni-NTA method

TAGZyme DAPase Enzyme removes dipeptides from N-terminal His tags expressed using TAGZyme pQE vectors with high efficiency and processivity.

The TAGZyme DAPase Enzyme - a recombinant exopeptidase carrying a His tag at its C-terminus - is removed from reaction mixtures by subtractive immobilized-metal affinity chromatography (IMAC), enabling pure, detagged target protein to be recovered in the flow-through fraction.

Feature	Specifications
Applications	Production of therapeutic proteins, protein structure determination using NMR or X-ray crystallography
Efficiency of Tag removal	>95%
Protease recognition site	Various signals (please see handbook)
Special feature	Highly specific exoproteolytic activity
Tag removal	Exoproteolytic
Time	30 minutes

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