QIAGEN

Strep-Tactin Superflow Plus

For batch, gravity-flow, or FPLC purification of Strep-tagged proteins

·         Three-fold increase in binding capacity (up to 9 mg/ml)

·         Ideal for purification from prokaryotic and eukaryotic systems

·         Enables automated purification on any liquid chromatography system

·         Minimal influence on protein structure, function, and immunogenicity

The small Strep-tag II (WSHPQFEK) can be used to purify active, native conformation proteins to high purity on Strep-Tactin resins. In addition, it can be used in combination with the His tag in a standardized two-step purification procedure.

Strep-Tactin is available coupled to magnetic beads for small-scale purification procedures (up to 100 ?g) or Superflow chromatography resin for larger scale purification (up to 9 mg/ml resin).

Strep-Tactin resin is also available in prefilled cartridges for automated purification on liquid chromatography systems.

Feature	Specifications
Applications	Proteomics
Binding capacity	up to 9 mg/ml
N- or C-terminal tag	Both
Processing	manual/automated
Scale	Strep-Tactin Superflow Plus: Large Scale; Strep-Tactin Superflow Plus Cartridge: medium scale
Start material	cell lysates
Support/matrix	Superflow Plus
Tag	Strep-tag
Yield	up to 9 mg/ml

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