QIAGEN

RNeasy Protect Mini Kit

For stabilization and purification of up to 100 µg total RNA from tissues

·         Immediate RNA stabilization and protection

·         Reliable gene expression and gene-profiling data

·         No need for liquid nitrogen, dry ice, or phenol

·         Ready-to-use, high-quality total RNA in minutes

·         No CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Protect Mini Kit includes RNAlater RNA Stabilization Reagent for stabilizing RNA in tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology.

RNA purification can be automated on the QIAcube.

Efficient disruption of tissue samples can be achieved using a TissueRuptor or TissueLyser system.

Feature	Specifications
Applications	PCR, qPCR, real-time RT-PCR, microarray
Elution volume	30-100 µl
Format	Spin column
Main sample type	Tissue samples
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Sample amount	0.5-30 mg
Stabilization	Yes
Technology	Silica technology
Yield	10 -45 µg

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