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Biologa Molecular .

RNeasy Mini Kit

QIAGEN

RNeasy Mini Kit


For purification of up to 100 g total RNA from cells, tissues, and yeast


  • Fast procedure delivering high-quality total RNA in minutes
  • Ready-to-use RNA for high performance in any downstream application
  • Consistent RNA yields from small amounts of starting material
  • No phenol/chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
The RNeasy Mini Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane RNeasy spin columns with a binding capacity of 100 g RNA.
Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNA purification using the RNeasy Mini Kit can be fully automated on the QIAcube.

For smaller and larger samples, the RNeasy Micro Kit (spin-column binding capacity of up to 45 g RNA), the RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

Feature

Specifications

Applications

Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing

Elution volume

30-100 l

Format

Spin column

Main sample type

Tissue, cells

Processing

Manual (centrifugation)

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

Total RNA

Sample amount

0.5-30 mg

Technology

Silica technology

Time per run or per prep

20 minutes

Yield

Varies


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