QIAGEN

RNeasy Micro Kit

For purification of up to 45 µg total RNA from cell and tissue samples

·         Fast procedure delivering high-quality total RNA in minutes

·         Ready-to-use RNA for high performance in any downstream application

·         Consistent RNA yields from very small amounts of starting material

·         No phenol/chloroform extraction

·         No CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Micro Kit is designed for purification of up to 45 ?g RNA from small cell and tissue samples.

Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

RNA purification using the RNeasy Micro Kit can be fully automated on the QIAcube.

For larger samples, the RNeasy Mini Kit (spin-column binding capacity of 100 µg RNA), RNeasy Midi Kit (spin-column binding capacity of 1 mg RNA), and RNeasy Maxi Kit (spin-column binding capacity of 6 mg RNA) are also available.

Feature

Specifications

Applications

Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing

Elution volume

10-14 µl

Format

Spin column

Main sample type

Tissue, cells

Processing

Manual

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

RNA

Sample amount

<5 mg

Technology

Silica technology

Time per run or per prep

30-40 minutes

Yield

Varies

Servicios

Equipo laboratorio

Material laboratorio

Audio y Video

Material de curación

Servicio Técnico

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