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Biología Molecular .

RNeasy Lipid Tissue Mini Kit

QIAGEN

RNeasy Lipid Tissue Mini Kit


For purification of up to 100 µg total RNA from fatty tissues and other types of tissue


  • Optimized lysis conditions for fatty tissues and other tissues
  • High yields of total RNA without phenol contamination
  • High-quality RNA for all downstream applications
The RNeasy Lipid Tissue Mini Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA.

The kit can be automated using the QIAcube. Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

For larger samples, the RNeasy Lipid Tissue Midi Kit (spin-column binding capacity of 1 mg RNA) is also available.

Feature

Specifications

Applications

PCR, real-time PCR, microarray

Elution volume

30-100 µl

Format

Spin column

Main sample type

Fatty tissue samples

Processing

Manual

Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein

RNA

Sample amount

10-100 mg

Technology

Silica technology

Time per run or per prep

45 minutes

Yield

2.4-10 mg


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