QIAGEN

RNeasy Lipid Tissue Midi Kit

For purification of up to 1 mg total RNA from fatty tissues and other types of tissue

·         Optimized lysis conditions for fatty tissues and other tissues

·         High yields of total RNA without phenol contamination

·         High-quality RNA for all downstream applications

The RNeasy Lipid Tissue Midi Kit includes QIAzol Lysis Reagent for lysing fatty tissues and other types of tissue, and RNeasy spin columns for purifying up to 1 mg of high-quality RNA.

Tissue samples can be conveniently stabilized using RNAlater RNA Stabilization Reagent (nonfatty tissues only) or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system.

For smaller samples, the RNeasy Lipid Tissue Mini Kit (spin-column binding capacity of 100 µg RNA) is also available.

Feature	Specifications
Applications	PCR, real-time PCR, microarray
Elution volume	150-500 µl
Format	Spin column
Main sample type	Fatty tissue samples
Processing	Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA
Sample amount	75-500 mg
Technology	Silica technology
Time per run or per prep	60 minutes
Yield	21-110 µg

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