QIAGEN

RGS·His Antibody, BSA-free

For sensitive detection of His-tagged proteins with an RGS·His epitope

· Highly specific detection of C-terminal, N-terminal, and internal His tags

· Highly sensitive and specific detection of RGS·His epitopes

Monoclonal mouse Anti·His Antibodies, like the RGS·His Antibody, BSA-free, are used to detect recombinant protein carrying His tags. RGS·His Antibody is one of three variants, each of which detects a different His tag epitope.

Like all QIAGEN mouse monoclonal antibodies, RGS·His Antibody, BSA-free, is prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.

Feature	Specifications
Detection	Secondary antibody required
Epitope detected	RGSHHHH
Sensitivity in dot blots -chemiluminescent substrate-	10 pg
Sensitivity in dot blots -chromogenic substrate-	0.5 ng
Sensitivity in western blots - Chemiluminescent substrate	50 pg
Sensitivity in western blots -chromogenic substrate-	2 ng
Substrate for blot detection	Dependent on secondary antibody
Substrates for assay procedure	Dependent on secondary antibody
Tag	His tag

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