QIAGEN

RGS·His Antibody

For sensitive detection of His-tagged proteins with an RGS·His epitope

· Highly specific detection of C-terminal, N-terminal, and internal His tags

· Highly sensitive and specific detection of RGS·His epitopes

Monoclonal mouse Anti·His Antibodies, like the Anti·His Antibody, are used to detect recombinant protein carrying His tags.

RGS·His is one of three variants, each of which detects a different His tag epitope.

Like all QIAGEN mouse monoclonal antibodies, RGS·His is prepared under serum-free conditions, which guarantee the absence of viruses, mycoplasma, and contaminating

Feature	Specifications
Detection	Secondary antibody required
Epitope detected	RGSHHHH
Sensitivity in dot blots -chemiluminescent substrate-	10 pg
Sensitivity in dot blots -chromogenic substrate-	0.5 ng
Sensitivity in western blots - Chemiluminescent substrate	50 pg
Sensitivity in western blots -chromogenic substrate-	2 ng
Substrate for blot detection	Dependent on secondary antibody
Substrates for assay procedure	Dependent on secondary antibody
Tag	His tag

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