QIAGEN

REPLI-g WTA Single Cell Kit

For whole transcriptome amplification of total RNA or mRNA from single cells

·         Complete transcriptome coverage from just single cells

·         Uniform WTA with negligible sequence bias due to MDA technology

·         Optimized for use with new technologies, including NGS

·         Amplification of total RNA or mRNA-enriched (poly A+) RNA

·         Novel tool for cancer and stem cell research

The REPLI-g WTA Single Cell Kit enables reliable investigation of effects on transcription regulation at the single-cell transcriptome level and allows uniform amplification of all transcripts from just single cells (1-1000 cells).

Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method.

The innovative lysis buffer effectively stabilizes cellular RNA, ensuring the resulting RNA accurately reflects the in vivo gene expression profile.

All enzymatic steps have been developed to enable efficient processing of RNA for accurate amplification of cDNA, which is achieved with negligible sequence bias using innovative Multiple Displacement Amplification (MDA) technology.

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