·
WGA from single cell material with complete genome
coverage
·
Unbiased amplification of genomic loci due to MDA
technology
·
Optimized for use with new technologies, including
NGS
·
Consistent yields of up to 40 µg (average product length
>10 kb)
·
Novel tool for cancer research, stem cell research, or
metagenomics
DNA sequence analysis and genotyping of biological
samples using innovative instrumentation, such as next-generation
sequencing (NGS) platforms, is often limited by the small amount
of sample available.
The REPLI-g Single Cell Kit is
specially designed to uniformly amplify genomic DNA from single cells
(1 to <1000 bacterial or tumor cells) or purified genomic DNA
with complete genome coverage.
Additional protocols are also available for use with
fresh or dried blood or fresh or frozen tissue. Dedicated buffers
and reagents undergo a unique, controlled decontamination procedure to
avoid amplification of contaminating DNA, ensuring highly reliable
results every time.
Accurate amplification of genomes with negligible
sequence bias and no genomic drop-outs is achieved with
innovative Multiple Displacement Amplification (MDA) technology. In
contrast to PCR-based WGA technologies, high fidelity rates are
increased up to 1000-fold, avoiding costly false positive or negative
results.
For more information about WGA technology, visit our
whole genome amplification Web resource, and for the specifications of
this, and other, dedicated REPLI-g products, refer to
Table 4.
