·
Easy amplification with consistent yields of 40
µg
·
Unbiased amplification of genomic
loci
·
Reliable results due to Multiple Displacement
Amplification (MDA)
·
Amplified DNA highly suitable for most downstream
applications
·
No risk of DNA degradation during long-term
storage
The REPLI-g Midi Kit provides optimized
reagents for whole genome amplification (WGA) from small samples using
innovative Multiple Displacement Amplification (MDA) technology.
The
typical DNA yield of a 50 ?l
reaction is up to 40 ?g,
with an average product length greater than 10 kb (ranging between 2
kb and 100 kb).
Unique REPLI-g technology delivers highly uniform WGA
from a variety of small or precious sample types, including purified
genomic DNA, or directly from fresh or dried blood, buccal swabs,
fresh or frozen tissue, and cells.
This simple
and reliable method is capable of accurate and unbiased amplification
of genomes and generates DNA that can be applied without further
purification or quantification for downstream applications that do not
require labeling.
In contrast to PCR-based WGA technologies, high fidelity
rates are increased up to 1000-fold, avoiding costly false positive or
negative results. For more information about WGA technology, visit our
WGA resource page, and for other dedicated REPLI-g products, refer to
Table 1.
