QIAGEN

REPLI-g Cell WGA & WTA Kit

For parallel whole genome and whole transcriptome amplification from cells and limited samples

·         Parallel amplification of genomic DNA and RNA (total or mRNA-enriched)

·         Easily correlate genomic status with transcription pattern

·         Negligible sequence bias due to MDA technology

·         Amplify gDNA and RNA directly from cells or tissue

·         For use with all downstream applications, including NGS

The REPLI-g Cell WGA & WTA Kit uniquely enables uniform whole genome amplification (WGA) and whole transcriptome amplification (WTA) in parallel reactions, allowing direct correlation of the genome with the transcriptome from very small samples (25-1000 cells).

Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to block amplification of contaminating nucleic acids by the REPLI-g method. The innovative lysis buffer effectively stabilizes cellular nucleic acids, ensuring accurate representation of both genomic DNA and RNA levels.

Unbiased amplification of genomic DNA and total RNA or mRNA (poly A+) is achieved using innovative Multiple Displacement Amplification (MDA) technology and the novel REPLI-g SensiPhi DNA Polymerase.

In contrast to PCR-based amplification technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

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