|
Feature |
Specifications |
|
Altitude |
Up to 2000 m (6500 ft.) |
|
Elution volume |
QIAsymphony SP — 50–400 µl
(application/protocol dependent) |
|
Features |
Rotor-Gene Q — dynamic range, 10 orders of
magnitude |
|
Heat dissipation/thermal load |
Rotor-Gene Q — average, 0.183 kW (632
BTU/hour); peak, 0.458 kW (1578 BTU/hour) |
|
Humidity |
QIAsymphony SP/AS — relative humidity of 15–75%
(noncondensing); maximum 75% relative humidity for temperatures up
to 31°C, decreasing linearly to 50% humidity at 32°C Rotor-Gene Q — relative humidity of 10–75%
(noncondensing) |
|
Input volume |
QIAsymphony SP — 100–2000 µl
(application/protocol dependent) |
|
Instrument dimensions |
QIAsymphony SP/AS — QIAsymphony SP, 130 x 75 x
103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS
(integrated operation), 185 x 103 x 73 cm Rotor-Gene Q — width, 37 cm (14.6 in.); height,
28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth
(door open), 53.8 cm (21.2 in.) |
|
Kits or Application Packs designed for this
instrument |
QIAsymphony SP — QIAsymphony DSP Virus/Pathogen
Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits
(not available in all countries), Rotor-Gene Kits and other QIAGEN
real-time and end-point PCR kits Rotor-Gene
Q — artus QS-RGQ Kits (not available in all countries), RG SYBR
Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG
Probe RT-PCR Kit; RG Multiplex PCR Kit |
|
Operating temperature |
QIAsymphony SP/AS — 15–32°C (59–89.6ºF) Rotor-Gene Q — 18–30ºC (64–86ºF) |
|
Optical System |
Rotor-Gene Q — up to 6 channels spanning UV to
infra-red wavelengths; excitation sources: high energy
light-emitting diodes; detector: photomultiplier; acquisition time:
4 s; the software allows to create new excitation/detection
wavelength combinations |
|
Overvoltage category |
II |
|
Place of operation |
For indoor use only |
|
Pollution level |
QIAsymphony SP/AS — 2 Rotor-Gene Q — 2; Environmental class 3K2 (IEC
60721-3-3) |
|
Power |
QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz,
1400 VA, mains supply voltage are not to exceed 10% of nominal
supply voltages Rotor-Gene Q — 100–240 V AC,
50–60 Hz, 560 VA (peak); Power consumption 8 VA (standby); Mains
supply voltage fluctuations are not to exceed 10% of the nominal
supply voltages; F5a 250 V fuse |
|
Processing |
QIAsymphony SP/AS — automated |
|
Protocols/main application on this
instrument |
QIAsymphony SP — purification of DNA, RNA, and
bacterial and viral nucleic acids from a wide range of starting
materials QIAsymphony AS — PCR
setup Rotor-Gene Q — gene expression
analysis, microRNA detection, virus detection, SNP genotyping, SNP
genotyping, High Resolution Melt analysis (HRM) |
|
Samples per run; throughput |
QIAsymphony SP — 1–96 samples in batches of
24 Rotor-Gene Q — tubes 0.2 ml; strip tubes
0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples
per run using a Rotor-Disc 100 |
|
Software |
Rotor-Gene Q — Rotor-Gene Q software, supplied
on the provided installation CD |
|
Storage temperature |
QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in
manufacturer's package Rotor-Gene Q — 15 to
30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative
humidity (noncondensing); environmental class 1K2 (IEC
60721-3-1) |
|
Technology |
QIAsymphony SP — QIAGEN magnetic-particles
chemistry Rotor-Gene Q — real-time PCR
cycler |
|
Thermal performance |
Rotor-Gene Q — temperature range, 35 to 99ºC;
temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock
start); temperature resolution, ±0.02ºC (smallest programmable
increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp
rates, air), >15ºC/s heating, >20ºC/s cooling |
|
Transportation conditions |
QIAsymphony SP/AS — –25 to 70°C (–13ºF to
158ºF) in manufacturer's package Rotor-Gene
Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75%
relative humidity (noncondensing); environmental class 2K2 (IEC
60721-3-2) |
|
Typical run time |
Rotor-Gene Q — 40 cycles in 45 min with the
QIAGEN RG Kits (assay dependent) |
|
Warranty |
Rotor-Gene Q — 1 year on instrument; lifetime
guarantee on excitation LEDs |
|
Weight |
QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg;
QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5
kg (27.6 lb.) (standard configuration) |