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Biología Molecular .

QIAsymphony RGQ
QIAGEN

El QIAsymphony RGQ es la preparación integrada automatizado de muestras y preparacion de las pruebas, con transferencia manual de la unidad de deteccion, para todas las necesidades de pruebas moleculares. Puebla, Mexico, Labequim Qiagen

Para la purificación automatizada de ARN total o ARN total con miARN, a partir de tejidos o células

  • Flexibilidad sin precedentes
  • Optimización del flujo de trabajo y la utilidad
  • Facilidad de uso maximizado y conveniencia
  • Ensayos regulados y las pruebas de laboratorio desarrollados
  • Diseñado para la más amplia gama de aplicaciones

De QIAGEN solución de flujo de trabajo de primer nivel de muestra y resultado comprende el QIAsymphony SP para la preparación de la muestra, el QIAsymphony AS para la configuración del ensayo, y una amplia gama de ensayos listos para su uso en el tiempo real termociclador de PCR Rotor-Gene Q. QIAGEN continúa para expandir la serie QIAsymphony con nuevas funciones, menús de ensayo, y los flujos de trabajo.

Feature

Specifications

Altitude

Up to 2000 m (6500 ft.)

Elution volume

QIAsymphony SP — 50–400 µl (application/protocol dependent)

Features

Rotor-Gene Q — dynamic range, 10 orders of magnitude

Heat dissipation/thermal load

Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)

Humidity

QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Rotor-Gene Q — relative humidity of 10–75% (noncondensing)

Input volume

QIAsymphony SP — 100–2000 µl (application/protocol dependent)

Instrument dimensions

QIAsymphony SP/AS — QIAsymphony SP, 130 x 75 x 103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm 
Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)

Kits or Application Packs designed for this instrument

QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit

Operating temperature

QIAsymphony SP/AS — 15–32°C (59–89.6ºF)
Rotor-Gene Q — 18–30ºC (64–86ºF)

Optical System

Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations

Overvoltage category

II

Place of operation

For indoor use only

Pollution level

QIAsymphony SP/AS — 2
Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)

Power

QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 560 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5a 250 V fuse

Processing

QIAsymphony SP/AS — automated

Protocols/main application on this instrument

QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials 
QIAsymphony AS — PCR setup
Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)

Samples per run; throughput

QIAsymphony SP — 1–96 samples in batches of 24
Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100

Software

Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD

Storage temperature

QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package
Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)

Technology

QIAsymphony SP — QIAGEN magnetic-particles chemistry
Rotor-Gene Q — real-time PCR cycler

Thermal performance

Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling

Transportation conditions

QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package
Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)

Typical run time

Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)

Warranty

Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs

Weight

QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)


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