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QIAGEN

 

QIAGEN Plasmid Plus Mega Kit

 

 

For the fastest, most convenient purification of up to 2.5 mg transfection-grade plasmid DNA

 

·         Rapid purification of up to 12 samples in 40-50 minutes

·         Easy vacuum-based parallel processing

·         Low elution volumes for highly concentrated DNA

·         Very low endotoxin levels due to special wash buffer

·         Suitable for all applications

The QIAGEN Plasmid Plus Mega Kit enables ultrafast, large-scale purification of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 samples in parallel, reducing the need for hands-on time.

Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation.

The QIAGEN Plasmid Plus Mega Kit also features a novel wash buffer for endotoxin reduction.

 

Feature

Specifications

Applications

Transfection of most cell lines, cloning, enzymatic restriction, in-vitro translation/transcription, sequencing

Culture volume/starting material

500 ml

Final DNA concentration

? 1 µg/µl

Plasmid type

Plasmids, cosmids

Processing

manual (centrifugation and vacuum), parallel processing

Samples per run; throughput

1-12 samples per run

Technology

Advanced Silica technology (non-chaotrophic), Endotoxin reduction

Time per run or prep per run

40 min

Yield

up to 2,5 mg

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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