QIAGEN

QIAGEN Large-Construct Kit

For purification of up to 50 ?g BAC, PAC, and P1 DNA or up to 200 ?g cosmid DNA, free of genomic DNA

·         Efficient removal of genomic DNA contamination

·         Pure, transfection-grade large-construct DNA

·         Easy and simple procedure

The QIAGEN Large-Construct Kit provides gravity-flow, anion-exchange columns for purification of large-molecular-weight DNA.

A unique integrated ATP-dependent exonuclease digestion step ensures selective removal of contaminating genomic DNA.

The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection.

Feature	Specifications
Applications	Subcloning, transfection, sequencing etc.
Culture volume/starting material	<500 ml culture volume
Plasmid type	BAC, PAC, P1, cosmid DNA
Processing	Manual (centrifugation)
Samples per run; throughput	1 sample per run
Technology	Anion-exchange technology
Time per run or prep per run	280 min
Yield	<150 ug

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