QIAGEN

PhosphoProtein Purification Kit

For purification of phosphorylated proteins by affinity chromatography

·         Complete separation of phosphorylated and unphosphorylated proteins

·         One kit contains columns, buffers, reagents, and ultrafiltration columns

·         Cell-signaling studies without the need for radioactivity

The PhosphoProtein Purification Kit, which is based on affinity chromatography, delivers a complete separation of phosphorylated and unphosphorylated proteins from a cell lysate, and therefore facilitates investigation of the phosphorylation status of both entire cells and specific proteins.

In addition, the ratio of phosphorylated to unphosphorylated forms of proteins can easily be determined.

The drastic reduction of the complexity of each fraction is especially useful when studying proteins of low abundance. The PhosphoProtein Purification Kit includes columns, reagents, buffers, and Nanosep Ultrafiltration Columns for efficient concentration and desalting of protein fractions.

Feature	Specifications
Applications	SDS-PAGE, mass spectrometry
Binding capacity/yield	500 µg
Fractions isolated	Two fractions
Protein with post-translational modification	Phosphorylation
Sample size	~1 x 10e7 cells
Species	Eukaryotes
Start material	Cells

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