QIAGEN

Penta·His Antibody, BSA-free

For sensitive detection of His-tagged proteins

· Detection of C-terminal, N-terminal, and internal His tags

· Highly sensitive and specific detection of HHHHH epitopes

Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope.

Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.

Feature	Specifications
Detection	Secondary antibody required
Epitope detected	HHHHH
Sensitivity in dot blots (chemiluminescent detection)	10 pg
Sensitivity in dot blots (chromogenic detection)	0.5 ng
Sensitivity in Western blots (chemiluminescent detection)	50 pg
Sensitivity in Western blots (chromogenic detection)	2 ng
Substrate for blot detection	Dependent on secondary antibody
Substrates for assay procedure	Dependent on secondary antibody
Tag	His-tag

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QIAGEN Penta·His Antibody, BSA-free

For sensitive detection of His-tagged proteins

QIAGEN

Penta·His Antibody, BSA-free