QIAGEN

PAXgene Tissue miRNA Kit

For purification of microRNA and total RNA from tissues fixed and stabilized in PAXgene Tissue Containers

·         Integrated fixation, stabilization, and purification

·         Effective purification of miRNA and total RNA

·         High-quality miRNA from tissues

·         Preserved tissue morphology

Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA.

The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers.

Purification is carried out using silica-based RNA purification technology in a spin-column format.

Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality miRNA and total RNA for molecular analysis

Feature	Specifications
Applications	Northern blot analysis, RT-PCR and quantitative, real-time RT-PCR, microarray analysis
Elution volume	14-40 µl
Format	Spin column
Main sample type	Human tissue
Processing	Manual (centrifugation)
Sample amount	4 x 15 x 15 mm
Technology	Silica technology
Time per run	60 min + 15 min incubation/8 samples
Yield	Depends on tissue type and starting material (fixed or PFPE) PAXgene Fixed Paraffin Embedded.

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