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PAXgene Tissue RNA Kit

 

 

For purification of total RNA from tissues fixed and stabilized in PAXgene Tissue Containers

 

·         Integrated system for fixation, stabilization, and purification

·         High-quality RNA from tissues with preserved morphology

PAXgene Tissue is a new, crosslinking-free, tissue-fixation technology to enable both molecular and traditional pathology testing. It simultaneously stabilizes molecular content and preserves morphology.

Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathology studies as well as for subsequent purification of RNA, miRNA, and/or DNA.

The PAXgene Tissue RNA Kit provodes purification of total RNA from tissues fixed and stabilized in PAXgene Tissue Containers.

 Purification is carried out using silica-based RNA purification technology in a spin-column format. Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality RNA for molecular analysis.

 

Feature

Specifications

Applications

RT-PCR and quantitative, real-time RT-PCR, expression array and chip analysis, next-generation sequencing, cDNA synthesis, RNase and S1 nuclease protection, northern, dot, and slot blot analysis, primer extension

Elution volume

14-40 µl

Format

Spin column

Main sample type

Human tissue

Processing

Manual (centrifugation)

Sample amount

4 x 15 x 15 mm

Technology

Silica technology

Time per run

60 min + 15 min incubation/8 samples

Yield

Depends on tissue type and starting material (fixed or PFPE*)
* PAXgene Fixed Paraffin Embedded. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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