QIAGEN

PAXgene Blood miRNA Kit

For purification of miRNA and total RNA from whole blood

·         Integrated system for collection, stabilization, and purification

·         Effective purification of miRNA and total RNA

·         RNA stabilization for up to 3 days at 18-25°C

·         Stabilization for at least 50 months at -20°C or -70°C

·

The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood.

Purification is carried out using silica-based RNA purification technology in a spin-column format.

Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube.

Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.

Feature	Specifications
Applications	RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis
Elution volume	40 µl
Format	Spin column
Main sample type	Whole blood
Processing	Manual (centrifugation) or on QIAcube
Sample amount	2.5 ml
Technology	Silica technology
Time per run or per prep	90 minutes/12 samples
Yield	>3 µg (total RNA)

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