QIAGEN

Oligotex Direct mRNA Mini Kit

For mRNA purification from cells and tissues

·         High recovery of pure mRNA in as little as 30 minutes

·         No oligo-dT cellulose or ethanol precipitation

·         Flexibility for use with widely varying amounts of starting material

Oligotex Direct mRNA Kits enable purification of mRNA from cells and tissues.

Each Oligotex Kit includes Oligotex resin for binding poly A⁺ mRNA, and spin columns for washing and

eluting bound mRNA.

For purification of mRNA from total RNA and cleanup of poly A⁺ in vitro transcripts, Oligotex mRNA Kits are available

Feature	Specifications
Applications	PCR, real-time PCR, microarray, transfection..
Elution volume	20-100 µl
Format	Spin column
Main sample type	Tissue, cells
Processing	Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Poly A+ mRNA
Sample amount	100 mg/2 x 10e7
Stabilization	Yes
Technology	Polystyrene-latex particles
Time per run or per prep	30-50 minutes
Yield	>90% recovery

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