QIAGEN

Ni-NTA Superflow 96 BioRobot Kit

For automated, medium-scale purification of 6xHis-tagged proteins

·         Fully automated purification of up to 10 mg protein per well

·         High-purity protein free from cross-contamination

·         Purification under native or denaturing conditions

The Ni-NTA Superflow 96 BioRobot Kit provides automated purification of up to 600 µg recombinant protein from 96 samples in parallel (standard protocol, 5 ml culture volume per sample).

Cleared bacterial cell lysates flow onto a filter plate preloaded with Ni-NTA Superflow by the BioRobot workstation.

This unique 96-well metal-chelate affinity-chromatography module strongly and selectively binds His-tagged proteins.

Ready-to-run protocols are provided for purification under native or denaturing conditions.

Feature	Specifications
Applications	Proteomics
Bead size	60-160 µm
Binding capacity	5-20 mg/ml
Number of preps per run	1-96 samples per run
Processing	Automated
Scale	Medium scale
Special feature	Cross-contamination-free
Start material	Cell lysate
Support/matrix	Superflow
Tag	6xHis tag
Yield	15 µg - 4 mg

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