·
Up
to 300 ?g
His-tagged protein per column in as little as 15
minutes
·
Purification under native and denaturing
conditions
·
Up to 95% homogeneity in one
step
·
Ready-to-use spin columns for rapid automated or manual
processing
Ni-NTA silica combines Ni-NTA with a macroporous silica
support material optimized to suppress nonspecific hydrophobic
interactions.
The Ni-NTA Spin Columns provide Ni-NTA
silica in a convenient microspin format for easy preparation of
multiple samples in parallel.
They provide a simple method for functional screening of
engineered proteins, selection of clones expressing full-length
translation products, and comparison of expression levels. Each spin
column can purify up to 300 µg of His-tagged protein. Like all Ni-NTA
matrices, Ni-NTA spin columns can be used for one-step protein
purification under native or denaturing
conditions.
