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Biología Molecular .

Ni-NTA Magnetic Agarose Beads

QIAGEN

Ni-NTA Magnetic Agarose Beads


For high-throughput, micro-scale purification of His-tagged proteins and versatile magnetocapture assays using His tags


  • Directed presentation for enhanced signal-to-noise and reproducibility
  • Wide range of binding capacities by varying the number of beads
  • Effective screening procedures even with crude cell lysates
  • Ideal for study of biomolecular interactions
  • Options for full automation
Ni-NTA Magnetic Agarose Beads are magnetic particles coated with Ni-NTA Agarose affinity purification matrix.
They are used for immobilizing and purifying recombinant proteins carrying a His tag.
Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity.
Once proteins are bound, the beads can be precipitated using a magnet, washed, and proteins eluted in small volumes of buffer under native or denaturing conditions.

Feature

Specifications

Applications

Proteomics

Bead size

20-70 µm

Binding capacity

Up to 2 mg/ml suspension (5%)

Processing

Automated/manual

Scale

Micro scale

Special feature

High throughput (96-well)

Start material

Cell lysate

Support/matrix

Magnetic agarose beads

Tag

6xHis tag

Yield

<10 mg protein per column


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